BioNanoLip
This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No 839315.
Typical BAM micrographs (300 µm x 500 µm) of meibomian films from healthy donors (upper row) and from MGD donors (bottom row) at ≥ 15 mN/m. The upper row also presents a topographical surface plot to illustrate the thickness heterogeneity of the meibomian layers.
Fluorescence images of healthy MGS at different surface pressures, and from different probes and layers of the film (Rho reports for the amphiphilic layer; Per for the nonpolar lipid stratum of the duplex film).
Fluorescence images of diseased MGS at different surface pressures, and from different probes and layers of the film (Rho reports for the amphiphilic layer; Per for the nonpolar lipid stratum of the duplex film).
Publications
Georgiev GA, Gh MS, Romano J, Dias Teixeira KL, Struble C, Ryan DS, Sia RK, Kitt JP, Harris JM, Hsu KL, Libby A, Odrich MG, Suárez T, McKown RL, Laurie GW. Lacritin proteoforms prevent tear film collapse and maintain epithelial homeostasis. J Biol Chem. 2021 Jan-Jun;296:100070. DOI: 10.1074/jbc.RA120.015833. Epub 2020 Nov 21. PMID: 33187980; PMCID: PMC7948570.
Eftimov P, Yokoi N, Melo AM, Daull P, Georgiev GA. Interactions of Meibum and Tears with Mucomimetic Polymers: A Hint towards the Interplay between the Layers of the Tear Film. Int J Mol Sci. 2021 Mar 9;22(5):2747. DOI: 10.3390/ijms22052747. PMID: 33803116; PMCID: PMC7963170.
Eftimov P, Olżyńska A, Melcrová A, Georgiev GA, Daull P, Garrigue JS, Cwiklik L. Improving Stability of Tear Film Lipid Layer via Concerted Action of Two Drug Molecules: A Biophysical View. Int J Mol Sci. 2020 Dec 14;21(24):9490. DOI: 10.3390/ijms21249490. PMID: 33327408; PMCID: PMC7764870.
Ewurum A, Ankem A, Georgiev G, Borchman D. A spectroscopic study of the composition and conformation of cholesteryl and wax esters purified from meibum. Chem Phys Lipids. 2021 Aug;238:105088. DOI: 10.1016/j.chemphyslip.2021.105088. Epub 2021 May 7. PMID: 33965419; PMCID: PMC8620918.
Eftimov P, Ewurum A, Ankem A, Borchman D, Georgiev GA. Correlations between bulk and surface properties of meibomian lipids with alteration of wax-to-sterol esters content. Chem Phys Lipids. 2022 Jan;242:105163.DOI: 10.1016/j.chemphyslip.2021.105163. Epub 2021 Dec 2. PMID: 34864017.
This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No 839315.